Page 108 - Demo

Biotechnology
Biotechnology—Advanced Kits, continued
Restriction Enzyme Analysis of DNA—
Student Laboratory Kit
Big Idea 3, Investigation 9
In this experiment, restriction enzymes are introduced as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and multiple recognition sites. Separation by agarose gel electrophoresis of digests of lambda DNA will yield separate bands corresponding to the DNA fragments. Students estimate the size of the fragments and determine which restriction enzyme was used to digest the DNA. Kit includes detailed instructions and enough materials for eight gels. Electrophoresis apparatus, power supply, micropipets and tips, balance, microwave or hot plate, and a white light illuminator are required and available separately.
Materials: InstaStain® Blue Cards and FlashBlueTM DNA Stain, Ready- to-LoadTM DNA samples, calibrated pipet, UltraSpec–AgaroseTM, practice gel loading solution, disposable pipets and electrophoresis buffer (50X). Note: DNA is included as Ready-to-Load Quick StripTM connected tubes or individual 0.5- or 1.5-mL microcentrifuge tubes. While these samples are room temperature stable for up to one month after receipt, a refrigerator is recommended for long term storage.
Protein Electrophoresis—
Student Laboratory Kit
By: The Flinn Staff
Take agarose electrophoresis to the next level by separating and analyzing proteins. This advanced biotechnology kit provides stepwise instructions for extracting proteins from plant or animal tissues and visualizing them using electrophoresis. Kit includes special protein lysing, staining, and destaining solutions. Proteins obtained from smooth, cardiac, and skeletal tissue within one species allow students to see the variation in the type and quantity of proteins that may be expressed by the same genome. Protein tissue samples extracted from different classes of plants or animals reveal variation between species. Note that protein electrophoresis produces broad bands like chromo- somal DNA rather than discrete bands observed with DNA fragments.
Complete for 24 students working in groups of four. Electrophoresis equipment, power supply, microcentrifuge, and tissue samples are required, but not included in the kit.
PCR-Based DNA Fingerprinting
PCR—polymerase chain reaction—is an amazing technique used to “amplify” a mere trace of DNA into enough material to fully analyze. In this kit, students actually get to use this amazing modern technique! PCR amplification of simulated crime scene and suspect DNA is followed by separation of the PCR reaction products by electrophoresis. The non-human DNA is amplified using an easy- to-use PCR reaction pellet and premixed primer. Instructions for the “cycling” process, using either a thermal cycler or three water baths, are provided in the comprehensive instruction guide. The kit does not contain human DNA. A wonderful way to capture the science behind CSI shows!
Requires a thermal cycler or three water baths and a microcentri- fuge. Gel electrophoresis equipment is required to analyze the PCR samples. Supplied with a live materials card which must be returned two weeks before needed.
1-800-452-1261
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Restriction Enzyme Analysis of DNA— Student Laboratory Kit
Big Idea 3, Investigation 9 FB2043
Catalog No.
Description
Price/Each
FB2043
Restriction Enzyme Analysis of DNA— Student Laboratory Kit
$107.30
Protein Electrophoresis—Student Laboratory Kit FB1927
Catalog No.
Description
Price/Each
FB1927
Protein Electrophoresis—Student Laboratory Kit
$67.75
PCR-Based DNA Fingerprinting FB1892
Catalog No.
Description
Price/Each
FB1892
PCR-Based DNA Fingerprinting
$ 187.30
FB1891
Edvocycler
2095.90
BIOTECHNOLOGY—ADVANCED KITS continued on next page.