Page 122 - Demo

Botany
Botany—Living Algae Cultures
★MAINTENANCE: Algal cultures are shipped in clear, two- to eight-ounce poly- styrene jars. Immediately upon receipt, loosen caps and aerate cultures with clean pipet (included). Leave caps loosely in place to prevent contamination, yet permit air exchange. Cultures should receive diffuse light and be held in a temperature range of 18–22 °C. Maintain long-term cultures in clean culture dishes, flasks, or battery jars using an appropriate medium. For lighting, use two 48-inch, 40-watt fluorescent bulbs (plant grow-light or wide-spectrum) at a height of 12–18 inches. Begin with a light cycle of 16 hours on 8 hours off and reduce to 12 hours on, 12 hours off after 7–10 days. Subculture to fresh media every 6–8 weeks.
Green Algae
This division includes both microscopic and macroscopic forms found in a remarkable diversity of habitats. The green algae are very common in aquatic and terrestrial environments and as endosymbionts among lichens, protozoans, and cnidaria. Food is stored as starch and pigments in the division are chlorophylls a and b, carotenes, and xanthophylls. ★MAINTENANCE: Maintain green algae in moderate to high light. Recommended culture media are referenced for each species listed. Algae culture media (FB1820) is a Bristol's Modified Medium.
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Volvox
LM1055, etc.
Catalog No. Description Class Size Price/Each
LM1041 Chlamydomonas.Alsoclassifiedwiththeprotozoa.Unicellular, 30 $ 8.65
LM1042 biflagellate organism. First in the Volvicine line of evolution to colonial 100 25.65
organization and multicellularity.*†
LM1043 Chlorella. Small, spherical, single-celled algae. Common 30 8.65
LM1044 endosymbiont with Hydra and Paramecium species. Popular food 100 25.65
organism for aquatic invertebrates.*†
LM1045 Desmids. Several species mixed. Bilaterally symmetrical cells 30 10.15
LM1046 composed of mirror-image halves. Unicellular, colonial or filamentous 100 25.65
forms with conspicuous chloroplasts.†
LM1153 Eudorina. Colonies of 16-32 flagellated cells arranged in alternating 30 8.45 LM1154 rings.*† 100 23.20
LM1155 Gonium. Simplest of the colonial flagellates—flat 4–16 cell colonies of 30 8.85 LM1156 Chlamydomonas-like cells.*† 100 24.85
LM1047 Hydrodictyon. Commonly called the “water-net”; possesses a unique 30 8.65
LM1048 colonial morphology resembling an open-mesh net. Cells are large and 100 25.65
multinucleate. Common in ponds, lakes, and slow-moving streams.*
LM1049 Oedogonium. Unbranched filaments which grow anchored to solid 30 8.65
LM1050 substrates by a single holdfast cell. Also grows as an epiphyte on 100 25.65
aquatic angiosperms.*
LM1151 Pandorina. Colonies of 16-32 characteristically strawberry-shaped 30 8.85 LM1152 flagellated cells.*† 100 23.20
LM1051 Spirogyra. Filaments composed of cylindrical cells with large central 30 8.65
LM1052 vacuoles and distinctive ribbon-like spiral chloroplasts. Reproduce 100 25.65
sexually by conjugation.†
LM1053 Ulothrix. Unbranched filaments which grow on rocks and wood 30 8.65
LM1054 anchored by a specialized holdfast cell.*† 100 25.65
LM1055 Volvox. Large, phototactic colonies of Chlamydomonas-like cells 30 8.65 surrounded by a common gelatinous matrix. Reproduce asexually
LM1056 via the development of daughter colonies within the hollow sphere 100 25.65 of the parent colony. Culture in timothy hay infusion.
LM1157 Zygnema. Short cylindrical cells arranged in filaments; two distinctive 30 8.85 LM1158 star-shaped chloroplasts per cell.† 100 23.90
Gonium
LM1155, etc.
Giant Microbes®
Amoeba, Orange, Giant Microbe® AP8511
Heart Cell, Giant Microbe® AP8532
Euglena, Giant Microbe® AP8529
*Bristol’s Modified Medium †Pringsheim’s Soil Water
Red Algae
The Rhodophyta division of algae is the source of agar. Members of this group all contain phycoerythrin, phycocyanin, and allophycocyanin, which contribute to its red or blue color. Phycoerythrin absorbs blue/ green light so red algae are able to thrive at greater depths than green algae. Food is stored as grains of glycogen that stain pinkish-brown with iodine–potassium iodide solution.
★MAINTENANCE: Maintain red algae in low light. Recommended culture media is Pringsheim’s Soil Water.
Catalog No.
Description
Class Size
Price/Each
LM1252
Batrachospermum. Red algae. The complex arrangement of individ- ual cells make this a must for algae studies. Tear-drop shaped cells form fringed spheres along a central axis.
30
$8.85
BOTANY—LIVING ALGAE CULTURES continued on next page.
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