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Living Algae Cultures
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Botany—Living Algae Cultures, continued
Diatoms
Diatoms possess chlorophylls a and c and accessory carotenoids that are responsible for the characteristic coloration. Food is stored as the polysaccharide chrysolaminarin. Diatoms can be either unicellular or chain-forming with siliceous cell walls composed of two overlapping plates.
★MAINTENANCE: Culture diatoms under moderate to high light in Pringsheim’s Soil Water or Knop’s solution (K0003).
Euglena
Euglenoids have a proteinaceous secondary cell covering called a pellicle and most species possess pronounced eye-spots (stigma). Products of photosynthesis are stored as the glucose polymer paramy- lon. Euglenoids have chlorophylls a and b.
★MAINTENANCE: Maintain Euglena under moderate light in Pringsheim’s Soil Water supplemented with one-fourth of a pea cotyledon or Algae Culture Medium (FB1820).
Algae Sets
These sets include those species most widely studied. Two choices, described below, are available. Species listed are those typically included. Occasional substitutions may be necessary depending on availability of individual species. Note that sets include cultures from several of the major eukaryotic divisions of algae.
BOTANY—LIVING ALGAE CULTURES continued on next page.
Diatoms LM1037, etc.
Catalog No.
Description
Class Size
Price/Each
LM1037
Diatoms. Mixed freshwater species.
30
$ 9.70
LM1038
100
31.25
LM1253
Synura. Colonial golden-brown algae. Ovoid-shaped flagellated cells are all attached around a central point.
30
8.75
Euglena
LM1039, etc.
Catalog No.
Description
Class Size
Price/Each
LM1039
Euglena. Extremely active organism moves by means of single flagellum. Popular for behavioral studies involving responses to light, temperature and pH.
30
$ 8.65
LM1040
100
25.65
Catalog No.
Description
Class Size
Price/Each
LM1059
Algae Set—Mixed. Includes six types in one container. Typically: Gloeocapsa, diatoms, desmids, Spirogyra, Volvox and Chlamydomonas.
30
$18.70
LM1060
Algae Set—Individual. Includes five types in indi- vidual containers. Typically: Gloeocapsa, diatoms, desmids, Spirogyra and Volvox.
30
44.55
Algae Set— Individual LM1060
Prepare Culture Media for Algae
Bristol’s Modified Medium
Make six stock solutions, each with one of the following salts dissolved in 400 milliliters of water:
10 g sodium nitrate (NaNO3)
1 g calcium chloride (CaCl2 • 2H2O)
3 g magnesium sulfate (MgSO4 • 7H2O)
3 g potassium phosphate dibasic (K2HPO4)
7 g potassium phosphate monobasic (KH2PO4)
1 g sodium chloride (NaCl)
Take 10 mL of each stock solution and add to 900 mL of
distilled water. Next add one drop of 1% iron(III) chloride solution, 40 mL of Pringsheim’s soil-water extract and autoclave.
Pringsheim’s Soil Water
Select a rich garden soil which has not been recently fertilized. Add 300 g of the soil to a gallon jar. Fill three-fourths full with distilled water and then add one gram of calcium carbonate (CaCO3). Plug or cap jar loosely and steam for one hour per day on each of three consecutive days.
To culture Spirogyra, do not add calcium carbonate. To culture Euglena add one-fourth of a pea cotyledon before steaming. This will enhance growth. To culture diatoms, add 10–30 mg of sodium metasilicate (Na2SiO3 • 9H2O) to every liter of medium.
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