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Column Chromatography—Student Laboratory Kit

By: The Flinn Staff

Item #: AP4847

Price: $66.70

In the Column Chromatography Chemistry Student Laboratory Kit, students will extract pigments from tomato paste and spinach powder using column chromatography. You are what you eat! Many vegetables contain pigments that contribute to their color.

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Product Details

You are what you eat! Many vegetables contain carotenoids, chlorophylls and other pigments that contribute to their characteristic colors. Pigments, such as ß-carotene, make vegetables “good for us.” In this kit, students will extract pigments from tomato paste and spinach powder using column chromatography, an analytical separation method used by scientists to separate, isolate and purify organic and biological compounds. The order in which the pigments are extracted is based on their polarity. Reusable columns, alumina, solvents and instructions are provided so students can master the art of packing a column. Tomato paste and freeze-dried spinach powder are included. Detailed student instructions are clearly written and incorporate sections on chromatography theory, pigment structure and the role these pigments play in biochemistry.

Complete for 30 students working in pairs. Additional columns are available separately. Perform this experiment in a fume hood or well-ventilated lab.