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ELISA—Demonstration Kit

By: The Flinn Staff

Item #: FB1790

Price: $56.65

In Stock.

ELISA Demonstration Kit for biology and life science is a realistic simulation of the steps involved in this important medical diagnostic test. Observe interactions of antibodies.

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This item can only be shipped to schools, museums and science centers

Product Details

ELISA is an acronym for the enzyme-linked immunosorbent assay—a widely used test to screen patients for allergies or certain diseases. In this interactive demonstration, you will guide your students through a realistic simulation of the steps involved in this important medical diagnostic test. Students will learn about how antigens are bound to a support material and will observe interactions of simulated primary and secondary antibodies with the antigen, and each other, to produce positive color tests. A great way to see how basic biology principles are applied for medical purposes! Lab activity is a chemical simulation—kit contains no actual blood, blood products, or any possible infectious agents. Includes enough materials to complete the demonstration as written seven times. A hot plate or microwave is required to prepare the agar for the demonstration but is not provided.

Specifications

Materials Included in Kit: 
Agar, 14 g
Hydrochloric acid, 0.01 M, 250 mL
Phenol red indicator solution, 0.02%, 50 mL
Sodium hydroxide solution, 0.005 M, 250 mL
Bottle, Boston round, LDPE, 2 oz, 5
Dropper plug, LDPE, 18 mm, 5
Label, negative control solution
Label, positive control solution
Label, antibody solution
Label, sera solution
Label, wash solution
Nasal closure cap, PP, 18 mm, 5
Reaction plate, 24-well, 7


Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Analyzing and interpreting data

Disciplinary Core Ideas

HS-LS1.A: Structure and Function

Crosscutting Concepts

Cause and effect
Systems and system models

Performance Expectations

HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.
HS-LS1-2. Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.