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Bio-Rad Out of the Blue Genotyping Extension

Item #: FB2504 

Price: $234.02

Ships directly from the manufacturer.

Use CRISPR-Cas9 gene editing to edit the lacZ gene in E. coli. Visualize results with blue-white screening and confirm them by PCR. Deepen your understanding of CRISPR, its promise and risks, and the ethics behind its many applications. 

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This item can only be shipped to schools, museums and science centers

Other Options

Item# FB2505 FB2579 FB2444 FB2443 FB2332 FB2504 FB2676 FB2442 FB2333
Type CRISPR Kit CRISPR and Genotyping Extension CRISPR and Genotyping Extension plus Small Fast Blast DNA Electrophoresis Pack CRISPR and Genotyping Extension with Small UView Electrophoresis Reagents CRISPR Refill Genotyping Extension Genotyping Extension with Small Fast Blast Electrophoresis Reagents Genotyping Extension with Small UView DNA Electrophoresis Pack Genotyping Reagent Refill
Price $305.26 $489.16 $562.72 $587.24 $243.97 $234.02 $317.52 $342.04 $219.45
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Product Details

Perform true CRISPR gene editing in your laboratory class!
CRISPR gene editing is revolutionizing scientific research. Now your students can do real CRISPR-Cas9 gene editing in the classroom using Bio-Rad's educational CRISPR gene editing kits.

  • Out of the Blue CRISPR Kit (FB2505) — apply CRISPR-Cas9 technology to disrupt the lacZ gene in E. coli and produce a phenotype that can be readily visualized through blue-white screening
  • Out of the Blue CRISPR Genotyping Extension (FB2504) — (optional) confirm the edit at the molecular level using PCR and agarose gel electrophoresis

The Out of the Blue CRISPR and Genotyping Extension Kits earned the 2020 Science Educators’ Best of Show award for “Best New Technology Innovation for Science”  from the National Science Teaching Association (NSTA).

Features and Benefits

  • Hands-on, true CRISPR gene editing — students get direct experience with this powerful technology
  • Visible phenotype — results can be observed with the naked eye in as little as 22 hr
  • Safe — familiar and common reagents and non-pathogenic, non-biofilm-forming bacteria generate a phenotype that is common to molecular biology
  • Accessible — does not require specialized equipment or techniques
  • Comprehensive, supportive curriculum — includes background about the components of a CRISPR-Cas9 system, the science — and math — behind its accuracy and flexibility, and the guided bioinformatic analysis of CRISPR targets students design themselves

 

Please note that, though some items may be shipped at ambient temperatures to reduce shipping costs, some items are to be stored refrigerated or frozen upon receipt to ensure longevity. 

Please store some components at 4°C or –20°C as indicated.

Specifications

CRISPR-Cas9 genotyping extension kit, includes Out of the Blue Extension Refill Pack materials (12012708EDU), InstaGene matrix, and PCR, micro, and conical tubes. Requires Out of the Blue CRISPR Kit (12012608EDU) and electrophoresis reagents, sold separately. For educational use only.

With the Out of the Blue Genotyping Extension Kit, students use multiplex PCR and agarose gel electrophoresis to confirm the edit they made to the lacZ gene with the Out of the Blue CRISPR Kit (sold separately). A key step in CRISPR and gene editing research workflows, this genotyping activity offers students an opportunity to engage in the scientific process of compiling evidence from multiple sources to explain a phenomenon.

Contents

Each kit contains the following materials for 8 student workstations (2–4 students per workstation).

Items to store at -20°C (components of the Out of the Blue Genotyping Extension Refill Pack, 12012708EDU):

  • 2x PCR Master Mix
  • Primer Mix
  • PCR Positive Control DNA
  • Molecular Weight Ruler
  • Orange G Loading Dye

Items to store at room temperature:

  • InstaGene Matrix
  • 1.5 ml Conical tubes with O-ring screw caps, 50
  • 1.5 ml Microcentrifuge tubes, 60
  • 0.2 ml PCR tubes, 100
  • Instructor Answer Guide

Bio-Rad Item No.: 12012607EDU