In the Plant Pigments and Photosynthesis Classic Laboratory Kits for AP®
Biology, separate and chromatograms and envision light reactions. Refill is available.
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First, students use thin-layer chromatography (TLC) to separate the pigments in blue-green algae and spinach then compare the chromatograms of the two diverse species. The second activity enables students to envision the light reactions as dye is reduced when it substitutes within the electron-transport chain. Fresh spinach, spectrophotometers, cuvets, a strong light source and a blender are required and available separately.
Materials: Dichlorophenolindophenol*, Acetone*, Aluminum foil,* Blue-green algae extract*, Cheesecloth*, Chromatography solvent*, Lens paper Parafilm*, Phosphate buffer concentrate*, Graduated pipets*, Thin-stem pipets*, Spinach extract*, 3-mL Syringes, 5-mL Syringes, Thin-layer chromatography sheets*
*Denotes items included with refill kit
Correlation to Next Generation Science Standards (NGSS)
Science & Engineering Practices
Planning and carrying out investigations
Analyzing and interpreting data
Using mathematics and computational thinking
Constructing explanations and designing solutions
Obtaining, evaluation, and communicating information
Disciplinary Core Ideas
HS-PS3.D: Energy in Chemical Processes
HS-LS1.A: Structure and Function
HS-LS1.C: Organization for Matter and Energy Flow in Organisms
HS-LS2.B: Cycle of Matter and Energy Transfer in Ecosystems
Cause and effect
Scale, proportion, and quantity
Energy and matter
Structure and function
Stability and change
HS-PS3-2. Develop and use models to illustrate that energy at the macroscopic scale can be accounted for as a combination of energy associated with the motion of particles (objects) and energy associated with the relative position of particles (objects).
HS-LS1-5. Use a model to illustrate how photosynthesis transforms light energy into stored chemical energy.
HS-LS1-6. Construct and revise an explanation based on evidence for how carbon, hydrogen, and oxygen from sugar molecules may combine with other elements to form amino acids and/or other large carbon-based molecules.
HS-LS2-4. Use mathematical representations to support claims for the cycling of matter and flow of energy among organisms in an ecosystem.