First, students use thin-layer chromatography (TLC) to separate the pigments in blue-green algae and spinach then compare the chromatograms of the two diverse species. The second activity enables students to envision the light reactions as dye is reduced when it substitutes within the electron-transport chain. Fresh spinach, spectrophotometers, cuvets, a strong light source and a blender are required and available separately.
Materials Included in Kit: Acetone, 50 mL Aluminum foil, 12" x 12" sheet Blue-green algae extract, 1 g Chromatography solvent, 80 mL Phosphate buffer concentrate, 100 mL Spinach powder, 1 g Cheesecloth, 1 sq yd Pipet, Beral-type, graduated, 32 Pipet, Beral-type, thin stem, 16 Parafilm, 2" wide x 12 feet long Thin layer chromatography sheet, 20 x 10 cm
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