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In the Sordaria Genetics Advanced Inquiry Lab Solution for AP® Biology, students prepare cross plates and cross wild-type and mutant strains of Sordaria fimicola, while observing the life cycle of a member of the fungal kingdom.
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Big Idea 3, Investigation 7
Students prepare cross plates and cross wild-type and mutant strains of Sordaria fimicola in this advanced genetics laboratory. Daily observation of the growing culture provides an opportunity to observe the life cycle of a member of the fungal kingdom. During meiosis, crossing over occurs in some of the asci. By counting the number of crossed ascospores versus uncrossed ascospores, it is possible to determine the distance between the gene for spore color and the centromere of the chromosome of interest. Students collect, prepare, count and calculate from their own cross plates.
Complete for 30 students working in pairs. S. fimicola wild-type and tan mutant strains are available available separately. An autoclave, stereoscopes, light microscopes and Bunsen burners are required.
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HS-LS3-1. Ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring.
HS-LS3-2. Make and defend a claim based on evidence that inheritable genetic variations may result from (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors.
HS-LS3-3. Apply concepts of statistics and probability to explain the variation and distribution of expressed traits in a population.