In the Properties of Lipids Biochemistry Laboratory Kit, examine the solubility of lipids and use a special “fat stain” to identify them.
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Fats and oils, waxes and cholesterol, steroid hormones and Vitamins A and D—what do these natural products have in common? All of these substances belong to the diverse class of biological compounds called lipids. What are lipids and what role do they play in our body chemistry? In this three-part student laboratory kit, students first examine the solubility of lipids and use Sudan III—a special “fat stain”—to identify lipids. Students then perform a test for unsaturation on a variety of seed oils to learn more about the vital role of saturated, unsaturated and polyunsaturated triglycerides in nutrition and health. Finally, students extract peanut oil from peanuts using hexane to determine the amount of fat in peanuts. This activity mimics the real-life processes used to study lipids, offering a stimulating link between biology, chemistry and nutrition, and allows students to compare their results with information provided on the nutritional labels of a variety of food items. Includes reproducible student handouts, detailed background information, Teacher Notes with sample data and answers to all questions, and all necessary chemicals and consumable supplies.
Complete for 30 students working in pairs. Perform this experiment in a fume hood or well-ventilated lab.
Correlation to Next Generation Science Standards (NGSS)
Science & Engineering Practices
Obtaining, evaluation, and communicating information
Planning and carrying out investigations
Constructing explanations and designing solutions
Analyzing and interpreting data
Disciplinary Core Ideas
HS-LS1.A: Structure and Function
HS-LS1.C: Organization for Matter and Energy Flow in Organisms
Cause and effect
Systems and system models
Structure and function
HS-LS1-2: Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.
HS-LS1-7: Use a model to illustrate that cellular respiration is a chemical process whereby the bonds of food molecules and oxygen molecules are broken and the bonds in new compounds are formed, resulting in a net transfer of energy.