In the Column Chromatography Chemistry Student Laboratory Kit, students will extract pigments from tomato paste and spinach powder using column chromatography. You are what you eat! Many vegetables contain pigments that contribute to their color.
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You are what you eat! Many vegetables contain carotenoids, chlorophylls and other pigments that contribute to their characteristic colors. Pigments, such as ß-carotene, make vegetables “good for us.” In this kit, students will extract pigments from tomato paste and spinach powder using column chromatography, an analytical separation method used by scientists to separate, isolate and purify organic and biological compounds. The order in which the pigments are extracted is based on their polarity. Reusable columns, alumina, solvents and instructions are provided so students can master the art of packing a column. Tomato paste and freeze-dried spinach powder are included. Detailed student instructions are clearly written and incorporate sections on chromatography theory, pigment structure and the role these pigments play in biochemistry.
Complete for 30 students working in pairs. Additional columns are available separately. Perform this experiment in a fume hood or well-ventilated lab.
Correlation to Next Generation Science Standards (NGSS)
Science & Engineering Practices
Developing and using models
Planning and carrying out investigations
Disciplinary Core Ideas
HS-PS1.A: Structure and Properties of Matter
HS-PS1.B: Chemical Reactions
HS-LS1.C: Organization for Matter and Energy Flow in Organisms
HS-ETS1.C: Optimizing the Design Solution
HS-PS3.D: Energy in Chemical Processes
HS-PS4.A: Wave Properties
Energy and matter
Cause and effect
Systems and system models
Structure and function
HS-LS1-5: Use a model to illustrate how photosynthesis transforms light energy into stored chemical energy.
HS-PS1-2: Construct and revise an explanation for the outcome of a simple chemical reaction based on the outermost electron states of atoms, trends in the periodic table, and knowledge of the patterns of chemical properties.
HS-PS3-5: Develop and use a model of two objects interacting through electric or magnetic fields to illustrate the forces between objects and the changes in energy of the objects due to the interaction.
HS-PS4-4: Evaluate the validity and reliability of claims in published materials of the effects that different frequencies of electromagnetic radiation have when absorbed by matter.