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Bio-Rad PV92 PCR Informatics Kit

Item #: FB2570 

Price: $268.00

Ships directly from the manufacturer.

Students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus. Using their results, they test the Hardy-Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.

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Options:

This item can only be shipped to schools, museums and science centers

Other Options

Item# FB2346 FB2570 FB2538 FB2537
Type Hair Follicle Preparation Reagent Kit Room-Temp Reagent Refill Temp-Sensitive Reagent Refill
Price $46.50 $268.00 $97.00 $139.00
Enter number of items

Product Details

Students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus. Using their results, they test the Hardy-Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.

Kit includes positive controls, PCR master mix, primers, extraction matrix, Fast Blast DNA stain, test tubes/holders, curriculum, and more for 32 students. PV92 PCR Kit TS (1662119EDU) and RT (1662139EDU)

Bio-Rad Item No.: 1662100EDU

View for storage recommendations and expanded kit info:
PV92 PCR Informatics Kit

 

Please note that, though some items may be shipped at ambient temperatures to reduce shipping costs, some items are to be stored refrigerated or frozen upon receipt to ensure longevity. 

Please store some components at 4°C or –20°C as indicated.

 

Specifications

With the PV92 PCR Informatics Kit, your students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus. Using their own results, students test the Hardy-Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.

Features and Benefits

  • Aligns with AP Biology Lab 8
  • Introduces PCR and applies it to population genetics

With this kit, students are able to:

  • Directly measure human diversity at the molecular level
  • Extract and amplify genomic DNA from their own samples
  • Compare results to online data

Background Information

In this activity, students hunt for a specific Alu repeat (a 300 base pair repetitive sequence of DNA) within a specific region called PV92 on chromosome 16. Over evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Some of us carry an Alu insertion and some of us do not. Such variations among individuals' genotypes are inherited — and are the raw material of genetic diversity and evolution. These subtle variations in our DNA are evidence of our ancestry, and form the basis of personal identification via DNA fingerprinting.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Preparing Cell Extracts: Two Convenient Options
           Option 1: Cheek Cell DNA Template Preparation
           Option 2: Hair Follicle DNA Template Preparation
Two robust methods for template preparation. For those instructors with local restrictions, we recommend the Hair Follicle DNA Template Preparation protocol.
Please note: the Hair Follicle DNA Template Preparation protocol requires the purchase of protease (Catalog No. FB2346)