Page 100 - Demo

Biotechnology
Biotechnology
Introductory Kits. . . . . . . . . . . . . . . . . . . . . . . 98–104 Advanced Kits. . . . . . . . . . . . . . . . . . . . . . . . 105–107 Equipment........................... 107–112
Biotechnology—Introductory Kits
Who’s the Daddy? DNA Profiling—Super
Value Kit
By: The Flinn Staff
Discover the power of DNA profiling (fingerprinting) using this creative simulation. No expensive equipment or chemicals needed! This activity only requires paper and scissors. Students solve a paternity case as they re-create each step of the DNA fingerprinting process. After assembling double-stranded DNA “molecules,” students cut them into fragments to simulate restriction enzyme digestion and then separate the fragments by size in a simulated gel. Next the DNA fragments are “denatured” (cut into single strands), “blotted” (pattern copied onto a sheet of paper), and treated with simulated radioactive probes that combine with only specific base sequences. The resulting simulated autoradiograph identifies target DNA fragments, which are used to match the unidenti- fied parent. This is a great teaching tool for students to learn how DNA fingerprinting really works! Includes black-line masters of simulated DNA segments, “radioactive probe” locators, electrophoresis gel blot- ter, magnetic tape, and zipper-lock bags are included.
Super Value Kit is complete for 30 students working in groups of six. All materials are reusable.
DNA Fingerprinting—Electrophoresis
at Work—Super Value Kit
By: The Flinn Staff
An introductory activity for students about to begin the study of biotechnology and electrophoresis. Students become medical forensic investigators as they read a case in which a woman has been attacked or a case in which an elderly man has been murdered. Once the students have read one of the case reports, they are provided with photo- reproductions of actual electrophoresis gels. Students will interpret the evidence against a list of possible suspects and attempt to solve the mystery. The design of the activity affords the instructor the flex- ibility to alter case outcomes from class to class and even manipulate evidence. Kit includes in-depth background material, preparation and instruction tips, student copies of case descriptions and investigation reports, and reproductions of evidence and suspect electrophoresis gels. The curiosity and excitement this activity creates will lead to a better understanding of electrophoresis and biotechnology. Time required for this activity is 30 minutes.
Super Value Kit is complete for 30 students working in pairs. All materials are reusable.
1-800-452-1261
flinnsci.com
Supplies............................. 112–114 DNA and Restriction Enzymes. . . . . . . . . . . 114–115
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Who’s the Daddy? DNA Profiling—Super Value Kit FB1758
DNA Fingerprinting—Electrophoresis at Work—Super Value Kit AB1370
Catalog No.
Description
Price/Each
FB1758
Who’s the Daddy? DNA Profiling—Super Value Kit
$20.40
Catalog No.
Description
Price/Each
AB1370
DNA Fingerprinting—Electrophoresis at Work— Super Value Kit
$56.75
BIOTECHNOLOGY—INTRODUCTORY KITS continued on next page.