Page 255 - Demo

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Viruses and Epidemiology
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Viruses and Epidemiology, continued
ELISA—Demonstration Kit
By: The Flinn Staff
ELISA is an acronym for the enzyme-linked immu-
nosorbent assay—a widely used test to screen patients for allergies or certain diseases . In this interactive demonstration, you will guide your students through a realistic simulation of the steps involved in this important medical diagnostic test . Students will learn about how antigens are bound to a support material and will observe interactions of simulated primary and secondary antibodies with the antigen, and each other, to produce positive color tests . A great way to see how basic biology principles are applied for medical purposes! Lab activity is a chemical simulation—kit contains no actual blood, blood products, or any possible infectious agents . Includes enough materials to complete the demonstration as written seven times .
Concepts: Antigen, antibody, ELISA, allergen .
Time Required: 25 minutes
Materials Provided: Antibody solution, phenol red indicator solution, negative control solution, positive control solution, sera solution, reac- tion plates, wash solution .
Note: A hot plate or microwave is required to prepare the agar for the demonstration but is not provided .
Antibody Function—
Student Laboratory Kit
By: William P. Baker, PhD
Western Kentucky Community and Technical College, Paducah, KY
The human body is constantly exposed to microbes, toxins, dust and pollen—foreign substances that are potentially harmful and may cause disease . One of the most important defenses the body uses to fight these foreign antigens is the production of antibodies . In this simulation of antibody diversity and antibody–antigen binding, students construct an antibody model by selecting from a variety of possible heavy and light chain regions to form an IgG antibody . They then investigate binding of their unique antibody with different antigen templates to determine if there is a match . Students subsequently understand the fate of their constructed antibody based upon it finding or not finding a matching antigen . Activity provides a creative way to identify “lock-and-key” biological relationships .
Complete for 30 students working individually.
Infection! Epidemic Simulation—
Super Value Laboratory Kit
By: The Flinn Staff
Have you ever been sick and wondered where you got it? Diseases may be spread by the exchange of body fluids, such as saliva, from one host to another . In this activity, the exchange of simulated saliva mimics the sharing of a beverage contaminated with an infectious virus . Students’ curiosity is piqued as they document the exchange partners in order to trace the infection and discover the original host .
Super Value Kit is complete for 5 classes of 30 students working individually.
ELISA— Demonstration Kit FB1790
Catalog No.
Description
Price/Each
FB1790
ELISA—Demonstration Kit
$56.65
Antibody Function— Student Laboratory Kit FB1967
Catalog No.
Description
Price/Each
FB1967
Antibody Function—Student Laboratory Kit
$18.75
Catalog No.
Description
Price/Each
FB2097
Infection! Epidemic Simulation— Super Value Laboratory Kit
$51.15
VIRUSES AND EPIDEMIOLOGY continued on next page.
Infection! Epidemic Simulation— Super Value Laboratory Kit FB2097