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Item #: FB2516 

Price: $218.74

Ships directly from the manufacturer.

Bio-Rad’s pGLO™ Bacterial Transformation Kits offer unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. They enable hands-on learning about the central dogma, genetic engineering, and the regulation of gene expression.

Please Note: Kit components may require refrigeration/freezer storage upon receipt. Please read kit instructions for more information. 

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This item can only be shipped to schools, museums and science centers

Other Options

Item# FB2517 FB2345 FB2565 FB2516 FB2354
Type pGLO Reagent Refill for FB2516 Transformation Reagent Refill for FB2565 and FB2354 Classic Kit AP Biology General Biology
Price $114.02 $83.37 $121.13 $218.74 $126.28
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Product Details

Bio-Rad’s pGLO Bacterial Transformation and GFP Kits offer engaging and unforgettable lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and glow green under UV light. These kits enable hands-on learning about the central dogma, gene expression and regulation, genetic engineering, protein separation, and the biomanufacturing process.

These lab kits are available in three formats to support your teaching needs:

  • pGLO Bacterial Transformation Kit (Classic) -- teach the basics of genetic engineering and the roles that external and internal factors play in gene regulation.
  • pGLO Bacterial Transformation Kit for General Biology -- teach core genetics concepts with a scaffolded learning approach
  • pGLO Transformation and Inquiry Kit for AP Biology -- students design their own inquiry investigations to examine the roles that external and internal factors play in bacterial transformation and gene regulation


Please note that, though some items may be shipped at ambient temperatures to reduce shipping costs, some items are to be stored refrigerated or frozen upon receipt to ensure longevity. 

Please store some components at 4°C as indicated.


Introducing pGLO Transformation and Inquiry for AP Biology: A ThINQ! Investigation, an inquiry-based kit designed to make students think.

Developed with master AP teachers, Bio-Rad's pGLO Transformation and Inquiry Kit for AP Biology includes five investigative labs in one kit to provide teachers with the tools and resources they need to guide students through the process of scientific discovery while developing their skills and gaining the knowledge that will help prepare them for success on the AP exam.

Students explore the process of bacterial transformation (genetic engineering) using Bio-Rad's exclusive pGLO plasmid. Then, you or your students choose to design and conduct a series of four additional inquiry investigations that explore the effects of changes in scientific design and challenge their understanding of the principles surrounding bacterial transformation.

Features and Benefits

By proceeding through multiple levels of inquiry exploration — from structured to guided to open inquiry — the pGLO Transformation and Inquiry Kit facilitates a new level of teaching and scientific discovery that engages students, promotes critical thinking and active learning, and encourages collaboration in the classroom.

The focus is not solely on achieving predetermined results; rather, the curriculum involves students making observations, raising questions, and designing scientific experiments to explore the world around them.

This AP-aligned curriculum includes the following:

  • Reagents, protocols, and background information for the classic pGLO Bacterial Transformation Lab plus four additional inquiry investigations
  • Prelaboratory activities that help students develop relevant and valid questions
  • Postlaboratory activities that use real-world data and ask students to analyze and discuss their findings
  • Essential knowledge, learning objectives, and science practices aligned with the 2012 AP curriculum to cover AP Big Ideas 1 and 3 and cross-cut to Big Ideas 2 and 4.
  • A successful teacher model process to assist you in implementing inquiry
  • A science case study that includes a relevant and engaging real-world story about bacterial transformation

How It Works

The kit curriculum is divided into several stages and levels of inquiry:

  • Bacterial transformation lab (structured inquiry) — this activity introduces the basics of bacterial transformation, data collection, and analysis of transformation efficiency. The variables that can affect transformation efficiency and other aspects of bacterial transformation are introduced and discussed, setting the stage for the series of four guided or open inquiry investigations
  • Inquiry investigation labs (guided or open inquiry) — these four guided and open inquiry labs are separate investigations; you choose which to perform and when. These activities extend the knowledge gained in the bacterial transformation lab as they guide students through designing their own experiments and communicating their results
    • Transformation efficiency: test variables and investigate their effects on transformation efficiency
    • Effect of ampicillin on bacterial growth: investigate the effects of different amounts of this antibiotic on bacterial growth
    • Effect of arabinose on GFP expression: investigate the effects of different amounts of this inducer on transgene expression
    • Satellite colonies: investigate the nature of satellite colonies, why they form and what they mean
  • Class discussion: science case study — the student case study connects the scientific knowledge students have gained with current issues in biological research that are related to bacterial transformation

Bio-Rad Item No.: 1660335EDU 

5-in-1 kit, includes reagents for transformation plus 4 inquiry investigations. 8 workstations of transformation, then 4 workstations of inquiry; 32 students. Teacher manual included