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Protein Electrophoresis—Student Laboratory Kit

By: The Flinn Staff

Item #: FB1927

Price: $67.75

In Stock.

Protein Electrophoresis Laboratory Kit for biotechnology provides stepwise instructions for extracting proteins from plant or animal tissues and visualizing them using electrophoresis.

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This item can only be shipped to schools, museums and science centers

Product Details

Take agarose electrophoresis to the next level by separating and analyzing proteins. This advanced biotechnology kit provides stepwise instructions for extracting proteins from plant or animal tissues and visualizing them using electrophoresis. Kit includes special protein lysing, staining, and destaining solutions. Proteins obtained from smooth, cardiac, and skeletal tissue within one species allow students to see the variation in the type and quantity of proteins that may be expressed by the same genome. Protein tissue samples extracted from different classes of plants or animals reveal variation between species. Note that protein electrophoresis produces broad bands like chromosomal DNA rather than discrete bands observed with DNA fragments. Enough materials are provided for 24 students working in groups of four. Electrophoresis equipment, power supply, microcentrifuge, and tissue samples are required but not included in the kit.

Specifications

Materials Included in Kit: 
Agarose, LE, 3 g
Gel loading solution, 6x, 10 mL
Protein destaining solution, 4x concentrate, 250 mL
Protein lysing buffer, 5x concentrate, 40 mL
Protein staining solution, 250 mL
Tris-glycine-sds buffer, 10x concentrate, 250 mL
Bags, reclosable, 4" x 8", 6
Microcentrifuge tube, 90
Pipet, Beral-type, graduated, 84
Pipets, needle tip, disposable, 36
Weighing dish, 5.5 g, 5½" x 5½", 6


Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Developing and using models
Planning and carrying out investigations
Analyzing and interpreting data
Engaging in argument from evidence

Disciplinary Core Ideas

HS-PS1.A: Structure and Properties of Matter
HS-LS1.A: Structure and Function

Crosscutting Concepts

Patterns
Cause and effect
Scale, proportion, and quantity
Systems and system models
Structure and function

Performance Expectations

HS-PS1-3. Plan and conduct an investigation to gather evidence to compare the structure of substances at the bulk scale to infer the strength of electrical forces between particles.
HS-PS3-5. Develop and use a model of two objects interacting through electric or magnetic fields to illustrate the forces between objects and the changes in energy of the objects due to the interaction.