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Roles of Restriction Enzymes—Student Laboratory Kit

By: The Flinn Staff

Item #: FB1960 

Price: $62.26

In Stock.

In the Roles of Restriction Enzymes Laboratory Kit for biotechnology, get a peek at restriction enzymes in action. Perfect combination of theory and practice.

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Product Details

Get a peek at restriction enzymes in action! Restriction enzymes recognize and cut DNA at specific sequences. First, students simultaneously run two identical DNA samples, one cut with EcoRI and the other cut with HindIII, using gel electrophoresis. Then the banding patterns produced are analyzed to see how the use of different restriction enzymes results in changes in the number and size of the bands. This lab helps students understand the theory of how restriction enzymes digest DNA. It also enhances laboratory skills such as preparing an agarose gel, loading and running DNA samples, and staining and destaining the gel to observe the separated bands of DNA. Perfect combination of theory and practice! Contains enough materials for a class of 24 students working in groups of four. Materials provided include sufficient agarose to make 6 gels, DNA samples cut with EcoRI and HindIII, respectively, TAE buffer, methylene blue for staining the gel, as well as needle-tip pipets and large weighing dishes. DNA source is Lambda phage.The DNA contained in this kit must be kept frozen prior to use. Orders will only be shipped between September 15 and May 15 unless we are told otherwise. Upon receipt, this kit should be stored in the freezer until just before it is to be used.


Materials Included in Kit: 
Agarose, LE, 3 g
Electrophoresis buffer, concentrated 50 x, 100 mL
λ DNA ecoRI digest, 80 μL, kept in freezer
λ DNA hindIII digest, 80 μL, kept in freezer
Methylene blue electrophoresis stain, 10x, 100 mL
Pipets, needle tip, disposable, 12
Weighing dish, disposable, 5.5 g, 5½" x 5½", 6

Correlation to Next Generation Science Standards (NGSS)

Science & Engineering Practices

Asking questions and defining problems
Planning and carrying out investigations
Analyzing and interpreting data
Engaging in argument from evidence

Disciplinary Core Ideas

HS-LS1.A: Structure and Function

Crosscutting Concepts

Cause and effect
Scale, proportion, and quantity
Structure and function
Stability and change

Performance Expectations

HS-LS1-1. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells.
HS-LS3-1. Ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions for characteristic traits passed from parents to offspring.