550 1-800-452-1261
Microbiology—Culture Supplies, continued
Gram Stain Set
Stains are formulated to meet the most widely accepted standards and are ready
to use. Includes crystal violet (Hucker), 100 mL; iodine, 100 mL; safranin, 100 mL;
95% ethanol (decolorizer), 500 mL; and one package of 20 thin-stem polyethylene
dropper pipets.
See free How To video at flinnsci.com.
Catalog No. Description Price/Each
FB0124 Gram Stain Set $30.95
Anti-Bacterial Soap
This is the professional anti-microbiological soap used by health care professionals
and medical facilities. 18 oz. Bottle has a built-in pump for easy dispensing at sink
locations.
Catalog No. Description Price/Each
FB1155 Anti-Bacterial Liquid Soap $20.65
Anti-Bacterial Soap
FB1155
MICROBIOLOGY continued on next page.
Gram Stain Set
FB0124
Protex™ Ultra Disinfectant Wipes
Protex™ Ultra Disinfectant Wipes eliminate 99.9% of bacteria in 15 seconds and
disinfect in just 4 minutes. Free of alcohol, bleach and solvents. 7" x 10". Package of 75.
Catalog No. Description Price/Pkg.
AP10032 Protex™ Ultra Disinfectant Wipes $14.95
Protex™ Ultra
Disinfectant Wipes
AP10032
Gram Stain
A powerful and widely used diagnostic tool of the microbiologist, this technique can be
easily and successfully practiced:
1. Thin smears of bacterial cultures to be tested should be air-dried and flame-fixed on
clean glass slides. 18- to 24-hour cultures will give the best results.
2. Apply Gram crystal violet stain from eye-dropper or dropper bottle. Slide should be
flooded with stain and let stand for one minute. Gently rinse slide with water from a
dropper or by dipping in clean water.
3. Flood smears with Gram iodine (serves as a mordant, or dye retainer) and let stand
for one minute. Rinse with clean water as above.
4. Decolorize smears with 95 percent ethyl alcohol, applying one drop at a time—until
no more color runs off—for about 30 seconds. Do not overdecolorize in this step. Rinse
with water as above.
5. Apply Gram safranin counterstain and let stand for 30–45 seconds. This step serves to
stain those bacteria (Gram-negative) not holding the crystal violet after step 4. Rinse
with water as above. Air or blot dry the slides and examine under oil-immersion.
Note: Take care not to allow any of the stains to evaporate to dryness on slides prior to
rinsing. Common spring-type wooden clothespins make excellent slide holders during
flaming, staining and rinsing procedures.
Dispose of Microbiological
Materials
Autoclaving is the best method for disposal of microbiological materials. All
materials should be autoclaved at 121 °C, 15 psi, for 15 to 60 minutes. (The
requirement for length of the autoclave run varies from state to state. Check
with your state department of health.) Biohazard bags can help simplify the
disposal procedure.
If you don’t have an autoclave (or a pressure cooker to autoclave) household
bleach or 70% ethanol can be used as an effective disinfectant. Dilute
full-strength bleach to 10% strength and then submerge cultures and other
potentially contaminated materials in the bleach or alcohol solution and let
sit overnight. When drained, place all materials into labeled trash bags for
disposal. See Biological Waste Disposal Section pages 1357–1358.
Clean Up
Microbiological Spills
1. Pour 70% isopropyl alcohol or 10% household bleach on any broken glass or contaminated
surfaces.
2. Cover spill area with paper towels, and saturate with additional alcohol or bleach
solution. Wait 30 minutes.
3. Wearing rubber gloves and using tongs, pick up all glass or residue along with the
paper towels and place into a Biohazard
bag.
4. Loosely close the biohazard bag, place it in the autoclave and sterilize the contents.
Follow safe disposal procedures for the biohazard bag.
5. Wash the spill area again with bleach or alcohol and wash hands thoroughly.
/flinnsci.com
/
/
/
/
/
/