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Microbiology—Media
Culture Media, Dehydrated
Highest quality dehydrated media for the culture of microorganisms. All are manufactured
in standard formulations under carefully controlled
conditions. To prepare
volumes of less than one liter, use an amount in proper proportion to that given in
the grams per liter column.
MICROBIOLOGY—MEDIA continued on next page.
Catalog No. Description
Package
Size/Grams
Grams/
Liter Liters/Pkg. Price/Each
A0084 Agar, Bacteriological Grade,
(Non-nutritive)
100 15 (plus
enrichments)
6.7 $ 21.25
A0085 500 33.0 62.90
D0049 Dextrose Agar 100 43 2.3 48.50
E0021 EMB Agar 100 36 2.8 55.95
M0209 MacConkey Agar 100 50 2.0 49.70
M0203 Mueller Hinton Agar* 100 38 13.1 40.55
N0092 Nutrient Agar 23 23 1.0 12.50
N0019 Nutrient Agar 100 23 4.3 41.00
N0020 500 21.7 110.00
N0021 Nutrient Broth 100 8 12.5 35.00
P0098 Potato Dextrose Agar 100 36 2.8 36.70
P0099 500 13.9 130.00
*Standard medium for antibiotic sensitivity testing.
†B0191 contains no blood.
Prepare
Dehydrated Media
When preparing dehydrated media for classroom use, there are several
important points to keep in mind:
• The preparation container should have a capacity 50 to 100 percent
larger than the final volume of media being prepared.
• Agar-based media should be heated slowly to the boiling point to
dissolve the agar. Stir or swirl frequently and avoid prolonged boiling.
• Large volumes of medium in a single container require extended sterilization
periods, which may cause deterioration. Uneven heating in a
large container could result in incomplete sterilization. Volumes to be
sterilized should not exceed one liter in a single container.
• Do not attempt to sterilize tightly closed containers. Containers should
be loosely capped or mouths covered with aluminum foil. Air and steam
must be allowed to pass freely into and out of the container.
• Liquid media should be slowly exhausted at the end of the sterilization
cycle. Pressure-cooker type sterilizers
should be removed from heat and
not opened until pressure indicator reads zero (ambient).
• Never autoclave/sterilize scratched or damaged glassware.
• Standard parameters for steam sterilization are 15 minutes at 15 pounds
of pressure (121 °C).
• As a guide: one liter of medium is sufficient to pour 50–60 standard (15
mm x 100 mm) Petri plates or 130–140 standard (16 mm x 150 mm) culture
tubes.
Culture Media, Prepared
Media prepared under carefully controlled conditions and inspected for sterility.
Tubes are prepared as slants in standard 16 x 125 mm screw-cap culture tubes.
Bottles contain 100 mL of media. Each bottle will pour 6–7 standard 15 x 100 mm Petri
plates or 15–16 culture tubes. Plates are 15 x 100 mm polystyrene; sealed to prevent
contamination and dehydration.
Catalog No. Description Package Price/Pkg.
FB1138 Blood Agar (TSA with 5% Sheep Blood) 10 plates $26.60
N0064 Nutrient Agar 6 tubes 17.55
N0077 3 bottles 23.50
FB0526 10 plates 24.50
T0085 Tryptic Soy Agar 3 bottles 25.50
FB0528 10 plates 27.00
NOTE: Culture media should be ordered as close as possible to the use date for optimal results.
Refrigerate upon receipt and use within one month. Not normally shipped during July and August.
Please specify shipping date.
BioPaddles®, Nutrient Agar, Pkg. of 10
FB2113
BioPaddles®
Ready to use and no refrigeration required! BioPaddles® have a longer shelf-life
than traditional Petri dishes and are very easy to use. Flexible dual-agar paddles are
enclosed in a sterile vial. Simply expose the media to the sample and then keep in
a warm place (35 °C).
Includes general instructions and access to detailed technical notes for each
paddle type. Nutrient agar paddles have nutrient agar media on both sides.
MacConkey/EMB paddles have MacConkey agar on one side and eosin methylene
blue agar on the other side. Use MacConkey agar to differentiate between Gram
negative bacteria while inhibiting the growth of most Gram positive bacteria. EMB
agar inhibits growth of Gram positive bacteria while cultivating growth of Gram
negative enteric microorganisms (bacilli). Package of 10 paddles.
Catalog No. Description Price/Each
FB2113 BioPaddles® Nutrient Agar, Pkg. of 10 $46.55
FB2114 BioPaddles® MacConkey and EMB Agar, Pkg. of 10 45.40
Tryptic Soy Agar
T0085
Nutrient Agar,
10 Plates
FB0526
CONTACT US VIA E-MAIL
FLINN@FLINNSCI.COM
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